A green-light-emitting, spontaneously blinking fluorophore based on intramolecular spirocyclization for dual-colour super-resolution imaging

Shin-nosuke Uno; Mako Kamiya; Akihiko Morozumi; Yasuteru Urano

doi:10.1039/C7CC07783A

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A green-light-emitting, spontaneously blinking fluorophore based on intramolecular spirocyclization for dual-colour super-resolution imaging†

Shin-nosuke Uno,

^a Mako Kamiya,

*^bc Akihiko Morozumi^a and Yasuteru Urano*^ab

Author affiliations

* Corresponding authors

^a Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
E-mail: uranokun@m.u-tokyo.ac.jp

^b Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
E-mail: mkamiya@m.u-tokyo.ac.jp

^c PRESTO, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan

Abstract

We have developed the first green-light-emitting, spontaneously blinking fluorophore (SBF), HEtetTFER. In combination with our near-infrared-light-emitting SBF (HMSiR), HEtetTFER allows dual-colour single-molecule localization microscopy (SMLM) in buffer solution without any additive and without photoactivation.

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Article information

DOI: https://doi.org/10.1039/C7CC07783A
Article type: Communication
Submitted: 08 Oct 2017
Accepted: 26 Nov 2017
First published: 07 Dec 2017

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Chem. Commun., 2018,54, 102-105

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A green-light-emitting, spontaneously blinking fluorophore based on intramolecular spirocyclization for dual-colour super-resolution imaging

S. Uno, M. Kamiya, A. Morozumi and Y. Urano, Chem. Commun., 2018, 54, 102 DOI: 10.1039/C7CC07783A

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Chemical Communications

A green-light-emitting, spontaneously blinking fluorophore based on intramolecular spirocyclization for dual-colour super-resolution imaging†

Abstract

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A green-light-emitting, spontaneously blinking fluorophore based on intramolecular spirocyclization for dual-colour super-resolution imaging

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