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Issue 8, 2018
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Surface-initiated atom transfer radical polymerization of cationic corona on iron oxide nanoparticles for magnetic sorting of macrophages

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Abstract

Ovalbumin-incorporated antigen carriers were prepared by surface-initiated atom transfer radical polymerization (SI-ATRP) of iron oxide nanoparticles for magnetic sorting of antigen-presenting cells. Iron oxide nanoparticles were surface-decorated with cationic shells by SI-ATRP, and the primary amines on the surface were further tri-methylated. Surface decoration of the nanoparticles was characterized by Fourier transform infrared spectroscopy, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, and transmission electron microscopy with energy-dispersive spectrometry. Ovalbumin-loaded nanoparticles showed higher incorporation in comparison to non-decorated nanoparticles, and the loaded ovalbumin was released faster at low pH than at neutral pH. Ovalbumin-loaded nanoparticles were endocytosed by macrophages, and macrophages with nanoparticles were easily harvested by magnetic separation. Magnetically sorted macrophages showed higher release of cytokines including TNF-α, MIP-1α, and IL-1β than unsorted macrophages. These results suggest that ovalbumin-loaded nanoparticles can potentially increase the efficiency of immune therapy during the antigen-presenting pathway.

Graphical abstract: Surface-initiated atom transfer radical polymerization of cationic corona on iron oxide nanoparticles for magnetic sorting of macrophages

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Publication details

The article was received on 17 Apr 2018, accepted on 28 Jun 2018 and first published on 28 Jun 2018


Article type: Paper
DOI: 10.1039/C8BM00418H
Biomater. Sci., 2018,6, 2248-2260

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    Surface-initiated atom transfer radical polymerization of cationic corona on iron oxide nanoparticles for magnetic sorting of macrophages

    M. K. Kang, W. Mao and H. S. Yoo, Biomater. Sci., 2018, 6, 2248
    DOI: 10.1039/C8BM00418H

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