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Issue 23, 2018
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A comparative study of branched and linear mannitol-based amphiphiles on membrane protein stability

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Abstract

The study of membrane proteins is extremely challenging, mainly because of the incompatibility of the hydrophobic surfaces of membrane proteins with an aqueous medium. Detergents are essential agents used to maintain membrane protein stability in non-native environments. However, conventional detergents fail to stabilize the native structures of many membrane proteins. Development of new amphipathic agents with enhanced efficacy for membrane protein stabilization is necessary to address this important problem. We have designed and synthesized linear and branched mannitol-based amphiphiles (MNAs), and comparative studies showed that most of the branched MNAs had advantages over the linear agents in terms of membrane protein stability. In addition, a couple of the new MNAs displayed favorable behaviors compared to n-dodecyl-β-D-maltoside and the previously developed MNAs in maintaining the native protein structures, indicating potential utility of these new agents in membrane protein study.

Graphical abstract: A comparative study of branched and linear mannitol-based amphiphiles on membrane protein stability

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Supplementary files

Article information


Submitted
25 Jul 2018
Accepted
04 Oct 2018
First published
18 Oct 2018

Analyst, 2018,143, 5702-5710
Article type
Paper

A comparative study of branched and linear mannitol-based amphiphiles on membrane protein stability

H. Hussain, T. Helton, Y. Du, J. S. Mortensen, P. Hariharan, M. Ehsan, B. Byrne, C. J. Loland, B. K. Kobilka, L. Guan and P. S. Chae, Analyst, 2018, 143, 5702
DOI: 10.1039/C8AN01408F

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