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Issue 20, 2018
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A ratiometric Raman probe for live-cell imaging of hydrogen sulfide in mitochondria by stimulated Raman scattering

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Abstract

Stimulated Raman Scattering (SRS) coupled with alkyne tags has been an emerging imaging technique to visualize small-molecule species with high sensitivity and specificity. Here we describe the development of a ratiometric Raman probe for visualizing hydrogen sulfide (H2S) species in living cells as the first alkyne-based sensor for SRS microscopy. This probe uses an azide unit as a selective reactive site, and it targets mitochondria with high specificity. The SRS ratiometric images show a strong response to H2S level changes in living cells.

Graphical abstract: A ratiometric Raman probe for live-cell imaging of hydrogen sulfide in mitochondria by stimulated Raman scattering

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Supplementary files

Article information


Submitted
16 May 2018
Accepted
31 Aug 2018
First published
03 Sep 2018

Analyst, 2018,143, 4844-4848
Article type
Communication

A ratiometric Raman probe for live-cell imaging of hydrogen sulfide in mitochondria by stimulated Raman scattering

C. Zeng, F. Hu, R. Long and W. Min, Analyst, 2018, 143, 4844
DOI: 10.1039/C8AN00910D

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