Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 4, 2018
Previous Article Next Article

Rapid resolution of carbohydrate isomers via multi-site derivatization ion mobility-mass spectrometry

Author affiliations

Abstract

Identifying small sugar isomers can be challenging by ion mobility-mass spectrometry (IM-MS) alone due to their small collision cross section differences. Herein, we report IM-MS results for multi-site, covalent carbohydrate derivatization with 3-carboxy-5-nitrophenylboronic acid (3C5NBA). Following reaction in aqueous solutions at room temperature, 3C5NBA reacts with each mono- or disaccharide molecule to yield products that each have a distinguishable mobility signature. The reaction was rapid and resulted in the detection of products within 5 min after 3C5NBA was mixed with the analyte. Eight disaccharides that varied in linkage, composition, and configuration (α or β) as well as four monosaccharides, D-glucose, D-galactose, D-mannose and D-fructose, were included in this study. The derivatives’ drift times showed significant shifts, with up to 3-fold gains in resolution when compared to previous literature reports. Moreover, the specific MS/MS fragmentation information gathered from these sugar derivatives provided further validation of the isomers’ structures.

Graphical abstract: Rapid resolution of carbohydrate isomers via multi-site derivatization ion mobility-mass spectrometry

Back to tab navigation

Supplementary files

Article information


Submitted
03 Nov 2017
Accepted
06 Jan 2018
First published
08 Jan 2018

Analyst, 2018,143, 949-955
Article type
Paper

Rapid resolution of carbohydrate isomers via multi-site derivatization ion mobility-mass spectrometry

L. Li, K. R. McKenna, Z. Li, M. Yadav, R. Krishnamurthy, C. L. Liotta and F. M. Fernández, Analyst, 2018, 143, 949
DOI: 10.1039/C7AN01796K

Social activity

Search articles by author

Spotlight

Advertisements