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Issue 35, 2017
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Biomaterials for polynucleotide delivery to anchorage-independent cells

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Abstract

Anchorage-independent cells possess morphological features and cell membrane compositions that are distinct from adherent cells. They display minimal surface area, have a low rate of endocytosis and generally possess few proteoglycans which make it a challenge to deliver nucleic acids into them. Wide ranges of methods and materials have been developed to tackle the delivery obstacles for the polynucleotide-based therapeutics in modifying non-adherent cells. This article summarizes the techniques and biomaterials that have been utilized for transfection of anchorage-independent cells. First, physical techniques are briefly described along with particular applications for which they are well-suited. The structure–activity relationship of various biomaterial carriers of polynucleotides are then discussed with strategies employed to enhance their capability to transfect anchorage-independent cells. In conclusion, the authors' perspectives on different methods for polynucleotide delivery to primary human cells are compared, along with a discussion of their progression towards clinical trials.

Graphical abstract: Biomaterials for polynucleotide delivery to anchorage-independent cells

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Article information


Submitted
07 Jul 2017
Accepted
09 Aug 2017
First published
09 Aug 2017

J. Mater. Chem. B, 2017,5, 7238-7261
Article type
Review Article

Biomaterials for polynucleotide delivery to anchorage-independent cells

A. S. Ansari, P. J. Santerre and H. Uludağ, J. Mater. Chem. B, 2017, 5, 7238
DOI: 10.1039/C7TB01833A

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