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Issue 23, 2017
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Tunable delayed controlled release profile from layered polymeric microparticles

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Abstract

Composite microparticles (MPs) with layered architecture, engineered from poly(L-lactic acid) (PLLA) and poly(D,L-lactic-co-glycolic acid) (PLGA), are promising devices for achieving the delayed release of proteins. Here, we build on a water-in-oil-in-oil-in-water emulsion method of fabricating layered MPs with an emphasis on modulating the delay period of the protein release profile. Particle hardening parameters (i.e. polymer precipitation rate and total hardening time) following water-in-oil-in-oil-in-water emulsions are known to affect MP structure such as the core/shell material and cargo localization. We demonstrate that layered MPs fabricated with two different solvent evaporation parameters not only alter polymer and protein distribution within the hardened MPs, but also affect their protein release profiles. Secondly, we hypothesize that ethanol (EtOH), a semi-polar solvent miscible in both the solvent (dichloromethane; DCM) and non-solvent aqueous phases, likely alters DCM and water flux from the dispersed oil phase. The results reveal that EtOH affects protein distribution within MPs, and may also influence MP structural properties such as porosity and polymer distribution. To our knowledge, we are the first to demonstrate EtOH as a means for modulating critical release parameters from protein-loaded, layered PLGA/PLLA MPs. Throughout all the groups in the study, we achieved differential delay periods (between 0–30 days after an initial burst release) and total protein release periods (∼30 to >58 days) as a function of solvent evaporation parameters and EtOH content. The layered MPs proposed in the study potentially have wide-reaching applications in tissue engineering for delayed and sequential protein release.

Graphical abstract: Tunable delayed controlled release profile from layered polymeric microparticles

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Supplementary files

Article information


Submitted
12 Jan 2017
Accepted
13 Mar 2017
First published
13 Mar 2017

J. Mater. Chem. B, 2017,5, 4487-4498
Article type
Paper

Tunable delayed controlled release profile from layered polymeric microparticles

D. Dutta, C. Fauer, K. Hickey, M. Salifu and S. E. Stabenfeldt, J. Mater. Chem. B, 2017, 5, 4487
DOI: 10.1039/C7TB00138J

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