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Issue 12, 2017
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Fabrication of cell-compatible hyaluronan hydrogels with a wide range of biophysical properties through high tyramine functionalization

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Abstract

Enzymatically mediated crosslinked hyaluronan–tyramine hydrogels (HA–Tyr) are promising matrices for tissue engineering and regenerative medicine. However, due to relatively low tyramine modifications of the hyaluronan backbone achieved, HA–Tyr matrices have a weak and narrow range of mechanical properties. The iterative use of 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM) as a coupling agent increased the yields of tyramine functionalization, which was reflected by a two-fold increase in Young's modulus of HA–Tyr hydrogels. The accurate control over hydrogel degradation was also facilitated. Viable encapsulation of human mesenchymal stem cells, with 85–98% over 6 days, was achieved in all hydrogels and distinct cellular spreading was observed in the absence of additional binding cues. The biophysical properties of the tunable HA–Tyr hydrogels are improved to study a wide range of cellular behaviors.

Graphical abstract: Fabrication of cell-compatible hyaluronan hydrogels with a wide range of biophysical properties through high tyramine functionalization

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Article information


Submitted
05 Dec 2016
Accepted
27 Feb 2017
First published
01 Mar 2017

J. Mater. Chem. B, 2017,5, 2355-2363
Article type
Paper

Fabrication of cell-compatible hyaluronan hydrogels with a wide range of biophysical properties through high tyramine functionalization

C. Loebel, T. Stauber, M. D'Este, M. Alini, M. Zenobi-Wong and D. Eglin, J. Mater. Chem. B, 2017, 5, 2355
DOI: 10.1039/C6TB03161G

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