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Issue 9, 2017
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Understanding single enzyme activity via the nano-impact technique

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Abstract

To evaluate the possible detection of single enzyme activity via electrochemical methods, a combined finite difference and random walk simulation is used to model individual enzyme-electrode collisions where such events are monitored amperometrically via the measurement of products formed by the enzyme in solution. It is found that the observed signal is highly sensitive to both the enzyme turnover number, the size of the electrode and the bandwidth of the electronics. Taking single catalase impacts as an example, simulation results are compared with experimental data. Our work shows the requirement for the detection of electrochemically active product formed by individual enzymes and gives guidance for the design of experiments.

Graphical abstract: Understanding single enzyme activity via the nano-impact technique

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Supplementary files

Article information


Submitted
09 May 2017
Accepted
17 Jul 2017
First published
19 Jul 2017

This article is Open Access
All publication charges for this article have been paid for by the Royal Society of Chemistry

Chem. Sci., 2017,8, 6423-6432
Article type
Edge Article

Understanding single enzyme activity via the nano-impact technique

C. Lin, E. Kätelhön, L. Sepunaru and R. G. Compton, Chem. Sci., 2017, 8, 6423
DOI: 10.1039/C7SC02084H

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