Issue 1, 2017

MnO2 nanosheet mediated “DD–A” FRET binary probes for sensitive detection of intracellular mRNA

Abstract

The donor donor–acceptor (DD–A) FRET model has proven to have a higher FRET efficiency than donor–acceptor acceptor (D–AA), donor–acceptor (D–A), and donor donor–acceptor acceptor (DD–AA) FRET models. The in-tube and in-cell experiments clearly demonstrate that the “DD–A” FRET binary probes can indeed increase the FRET efficiency and provide higher imaging contrast, which is about one order of magnitude higher than the ordinary “D–A” model. Furthermore, MnO2 nanosheets were employed to deliver these probes into living cells for intracellular TK1 mRNA detection because they can adsorb ssDNA probes, penetrate across the cell membrane and be reduced to Mn2+ ions by intracellular GSH. The results indicated that the MnO2 nanosheet mediated “DD–A” FRET binary probes are capable of sensitive and selective sensing gene expression and chemical-stimuli changes in gene expression levels in cancer cells. We believe that the MnO2 nanosheet mediated “DD–A” FRET binary probes have the potential as a simple but powerful tool for basic research and clinical diagnosis.

Graphical abstract: MnO2 nanosheet mediated “DD–A” FRET binary probes for sensitive detection of intracellular mRNA

Supplementary files

Article information

Article type
Edge Article
Submitted
18 Jul 2016
Accepted
01 Sep 2016
First published
05 Sep 2016
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY-NC license

Chem. Sci., 2017,8, 668-673

MnO2 nanosheet mediated “DD–A” FRET binary probes for sensitive detection of intracellular mRNA

M. Ou, J. Huang, X. Yang, K. Quan, Y. Yang, N. Xie and K. Wang, Chem. Sci., 2017, 8, 668 DOI: 10.1039/C6SC03162E

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements