A general [18F]AlF radiochemistry procedure on two automated synthesis platforms

Abstract

The aluminium fluoride-18 ([¹⁸F]AlF) radiolabelling procedure has generated great interest because it is a simple, one-pot method that can be used to directly radiolabel small molecules, peptides and proteins without the requirement for a [¹⁸F]fluoride drying step. Reported here is the development of an automated [¹⁸F]AlF radiolabelling procedure of three different precursors (one small molecule and two peptides) on two automated synthesis platforms: GE TRACERlab FX_FN and Trasis AllInOne (AIO). Aiming at the clinical translatability of a [¹⁸F]AlF radiosynthetic methodology, the use of both platforms yielded radioconjugates with >98% radiochemical purity (RCP) within 26–35 min and required a single rapid purification step. The Trasis AIO platform gave improved [¹⁸F]fluoride incorporation, and generally produced radioconjugates with a higher radiochemical yield (RCY) and effective specific activities (SA) when compared to the GE TRACERlab FX_FN system.