Issue 13, 2017

A fluorescent dye with large Stokes shift and high stability: synthesis and application to live cell imaging

Abstract

A fluorescent dye, 2,5-bis(6-amine-benzoxazol-2-yl)thiophene (BBTA), was synthesized by a two-step reaction from starting material 2,5-bis(benzoxazol-2-yl)thiophene (BBT). BBTA exhibited strong emission and large Stokes shift in solvent, and the largest Stokes shift (Δλ = 186 nm or Δν = 8572 cm−1) was obtained in buffer solution (PBS, pH = 7.2) with 5% (v/v) of polyethylene glycol monomethyl ether (mPEG550, MW = 550) as additive. Application of BBTA to live cell imaging showed that BBTA was clearly expressed in mitochondria with high contrast. Besides, BBTA showed low cytotoxicity and excellent photo-stability.

Graphical abstract: A fluorescent dye with large Stokes shift and high stability: synthesis and application to live cell imaging

Article information

Article type
Paper
Submitted
30 Nov 2016
Accepted
11 Jan 2017
First published
25 Jan 2017
This article is Open Access
Creative Commons BY license

RSC Adv., 2017,7, 7604-7609

A fluorescent dye with large Stokes shift and high stability: synthesis and application to live cell imaging

Z. Gao, Y. Hao, M. Zheng and Y. Chen, RSC Adv., 2017, 7, 7604 DOI: 10.1039/C6RA27547H

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