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Issue 15, 2017
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Mapping metabolites from rough terrain: laser ablation electrospray ionization on non-flat samples

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Abstract

Established laser-based ionization experiments require the surface of a sample to be as flat as possible to guarantee optimal laser focus. A laser ablation electrospray ionization (LAESI) source was custom-built to accommodate the topography of non-flat sample surfaces. Employing a confocal distance sensor, a height profile of the surface in question was recorded prior to the actual ionization experiment. The robustness of the system was evaluated by the metabolic profiling of radish (Raphanus sativus) leaves, chosen due to their pronounced surface features and known content of specialized metabolites. After the ionization experiments, light microscopy imaging was performed to evaluate ablation crater size and position. Reproducible ablation mark diameters of 69 ± 7 μm in average have been achieved. Mass spectrometric imaging capability has been proven on R. sativus leaf samples as well.

Graphical abstract: Mapping metabolites from rough terrain: laser ablation electrospray ionization on non-flat samples

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Article information


Submitted
15 Nov 2016
Accepted
12 Jan 2017
First published
27 Jan 2017

This article is Open Access

RSC Adv., 2017,7, 9045-9050
Article type
Paper

Mapping metabolites from rough terrain: laser ablation electrospray ionization on non-flat samples

B. Bartels, P. Kulkarni, N. Danz, S. Böcker, H. P. Saluz and A. Svatoš, RSC Adv., 2017, 7, 9045
DOI: 10.1039/C6RA26854D

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