Clean G-SERF an NMR experiment for the complete eradication of axial peaks and undesired couplings from the complex spectrum

Abstract

The G-SERF and other related experiments are very useful in the measurement of proton–proton couplings from the overlapped spectrum. However, they suffer from the presence of intense axial peaks and undesirable couplings in the spectra, thereby creating ambiguity in the assignment of peaks and subsequent measurement of ¹H–¹H couplings. In the present study we are reporting a new two dimensional NMR experiment cited as Clean-G-SERF, which is able to suppress all the axial peaks and unwanted couplings, while retaining only the couplings to the selectively excited proton. The method permits unambiguous assignment of peaks to the coupled partners of the selectively excited proton and enables the accurate measurement of couplings.