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Issue 10, 2017
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Fluorescent RAFT polymers bearing a nitrilotriacetic acid (NTA) ligand at the α-chain-end for the site-specific labeling of histidine-tagged proteins

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Abstract

Site-specific labeling of proteins is a valuable tool to study biological processes. We show that it can be achieved using fluorescent polymer probes synthesized by RAFT polymerization. Thanks to an original control agent functionalized with a nitrilotriacetic acid (NTA) ligand, an α-NTA polymer probe was prepared to selectively label histidine-tagged recombinant proteins.

Graphical abstract: Fluorescent RAFT polymers bearing a nitrilotriacetic acid (NTA) ligand at the α-chain-end for the site-specific labeling of histidine-tagged proteins

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Supplementary files

Article information


Submitted
23 Dec 2016
Accepted
02 Feb 2017
First published
03 Feb 2017

Polym. Chem., 2017,8, 1611-1615
Article type
Communication

Fluorescent RAFT polymers bearing a nitrilotriacetic acid (NTA) ligand at the α-chain-end for the site-specific labeling of histidine-tagged proteins

D. Duret, Z. Haftek-Terreau, M. Carretier, C. Ladavière, M. Charreyre and A. Favier, Polym. Chem., 2017, 8, 1611
DOI: 10.1039/C6PY02222G

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