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Issue 37, 2017
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A fluorescent pH probe for acidic organelles in living cells

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Abstract

A water-soluble pH sensor, 2-(6-(4-aminostyryl)-1,3-dioxo-1H-benzo[de]isoquinolin-2(3H)-yl)-N, N-dimethylethanamine (ADA), was synthesized based on the molecular design of photoinduced electron transfer (PET) and intramolecular charge transfer (ICT). The fluorescence emission response against a pH value is in the range 3–6, which is suitable for labelling intracellular pH-dependent microenvironments. After biological evolution, ADA is more than a pH biosensor because it is also an endocytosis pathway tracking biosensor that labels endosomes, late endosomes, and lysosome pH gradients. From this, the emissive aggregates of ADA and protonated-ADA in these organs were evaluated to explore how this probe stresses emission colour change to cause these unique cellular images.

Graphical abstract: A fluorescent pH probe for acidic organelles in living cells

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Supplementary files

Article information


Submitted
16 Aug 2017
Accepted
04 Sep 2017
First published
04 Sep 2017

Org. Biomol. Chem., 2017,15, 7936-7943
Article type
Paper

A fluorescent pH probe for acidic organelles in living cells

J. Chen, C. Chen and C. Chang, Org. Biomol. Chem., 2017, 15, 7936
DOI: 10.1039/C7OB02037F

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