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Issue 24, 2017
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Photo-controlled cell-specific metabolic labeling of RNA

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Abstract

Elucidating gene expression programs within a cell-specific manner is a grand challenge for biologists. Harder still is the ability to have kinetic control over such experiments. Metabolic labeling with bioorthogonally-functionalized metabolic intermediates provides a means to profile RNA expression in a cell-specific manner, but there is still a lack of kinetic resolution. Herein we present the synthesis and evaluation of photocaged metabolic uracil intermediates. We compare the photo-decaging properties and demonstrate their utility in metabolic labeling experiments in a cell-specific manner. We anticipate that our approach will have far-reaching impact as it provides control over tagging of nascent RNA.

Graphical abstract: Photo-controlled cell-specific metabolic labeling of RNA

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Supplementary files

Article information


Submitted
25 Apr 2017
Accepted
18 May 2017
First published
18 May 2017

Org. Biomol. Chem., 2017,15, 5117-5120
Article type
Communication

Photo-controlled cell-specific metabolic labeling of RNA

C. Feng, Y. Li and R. C. Spitale, Org. Biomol. Chem., 2017, 15, 5117
DOI: 10.1039/C7OB01009E

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