Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.

Issue 24, 2017
Previous Article Next Article

Photo-controlled cell-specific metabolic labeling of RNA

Author affiliations


Elucidating gene expression programs within a cell-specific manner is a grand challenge for biologists. Harder still is the ability to have kinetic control over such experiments. Metabolic labeling with bioorthogonally-functionalized metabolic intermediates provides a means to profile RNA expression in a cell-specific manner, but there is still a lack of kinetic resolution. Herein we present the synthesis and evaluation of photocaged metabolic uracil intermediates. We compare the photo-decaging properties and demonstrate their utility in metabolic labeling experiments in a cell-specific manner. We anticipate that our approach will have far-reaching impact as it provides control over tagging of nascent RNA.

Graphical abstract: Photo-controlled cell-specific metabolic labeling of RNA

Back to tab navigation

Supplementary files

Article information

25 Apr 2017
18 May 2017
First published
18 May 2017

Org. Biomol. Chem., 2017,15, 5117-5120
Article type

Photo-controlled cell-specific metabolic labeling of RNA

C. Feng, Y. Li and R. C. Spitale, Org. Biomol. Chem., 2017, 15, 5117
DOI: 10.1039/C7OB01009E

Social activity

Search articles by author