Imidazolium-labeled glycosides as probes to harness glycosyltransferase activity in human breast milk
Imidazolium-labeled (ITag-) glycosides are used to harness the glycosyltransferase activity directly from human breast milk. The covalently attached ionic labels provide a bifunctional chemical handle that is used to monitor reaction progress by MS, as well as aid in product purification from complex mixtures. The technology is exemplified in the synthesis of biologically relevant oligosaccharide analogs, LacNAc-ITag, ITag-Lewisx and ITag-Lewisa, in a matter of days from human breast milk without having to isolate specific enzymes.
- This article is part of the themed collections: Celebrating excellence in research: women of organic chemistry and 2017 Hot Articles in Organic and Biomolecular Chemistry