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Issue 13, 2017
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Biosynthetic 4,6-dehydratase gene deletion: isolation of a glucosylated jadomycin natural product provides insight into the substrate specificity of glycosyltransferase JadS

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Abstract

Deletion of the biosynthetic 4,6-dehydratase gene, jadT, present in the angucycline jadomycin dideoxysugar biosynthetic pathway, led to the isolation of a novel C12 glucosylated jadomycin. JadS was identified as the catalyst responsible for glucosylation due to a loss of production of the glucosylated natural product in a ΔjadSΔjadT deletion strain. This study demonstrates that a 2,6-dideoxy-L-sugar glycosyltransferase is able to transfer D-glucose, exemplifying remarkable substrate tolerance.

Graphical abstract: Biosynthetic 4,6-dehydratase gene deletion: isolation of a glucosylated jadomycin natural product provides insight into the substrate specificity of glycosyltransferase JadS

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Supplementary files

Article information


Submitted
02 Feb 2017
Accepted
16 Feb 2017
First published
16 Feb 2017

Org. Biomol. Chem., 2017,15, 2725-2729
Article type
Communication

Biosynthetic 4,6-dehydratase gene deletion: isolation of a glucosylated jadomycin natural product provides insight into the substrate specificity of glycosyltransferase JadS

S. M. Forget, J. Na, N. E. McCormick and D. L. Jakeman, Org. Biomol. Chem., 2017, 15, 2725
DOI: 10.1039/C7OB00259A

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