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Issue 13, 2017
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Biosynthetic 4,6-dehydratase gene deletion: isolation of a glucosylated jadomycin natural product provides insight into the substrate specificity of glycosyltransferase JadS

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Abstract

Deletion of the biosynthetic 4,6-dehydratase gene, jadT, present in the angucycline jadomycin dideoxysugar biosynthetic pathway, led to the isolation of a novel C12 glucosylated jadomycin. JadS was identified as the catalyst responsible for glucosylation due to a loss of production of the glucosylated natural product in a ΔjadSΔjadT deletion strain. This study demonstrates that a 2,6-dideoxy-L-sugar glycosyltransferase is able to transfer D-glucose, exemplifying remarkable substrate tolerance.

Graphical abstract: Biosynthetic 4,6-dehydratase gene deletion: isolation of a glucosylated jadomycin natural product provides insight into the substrate specificity of glycosyltransferase JadS

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Publication details

The article was received on 02 Feb 2017, accepted on 16 Feb 2017 and first published on 16 Feb 2017


Article type: Communication
DOI: 10.1039/C7OB00259A
Citation: Org. Biomol. Chem., 2017,15, 2725-2729

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    Biosynthetic 4,6-dehydratase gene deletion: isolation of a glucosylated jadomycin natural product provides insight into the substrate specificity of glycosyltransferase JadS

    S. M. Forget, J. Na, N. E. McCormick and D. L. Jakeman, Org. Biomol. Chem., 2017, 15, 2725
    DOI: 10.1039/C7OB00259A

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