Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 9, 2017
Previous Article Next Article

Whole cell-SELEX of aptamers with a tyrosine-like side chain against live bacteria

Author affiliations

Abstract

In an effort to expand the binding and recognition capabilities of aptamers, a nucleoside triphosphate modified with a phenol that mimics the side chain of tyrosine was used in the selection of DNA aptamers against live bacteria. Of multiple modified aptamers that were isolated against Escherichia coli DH5α cells, one aptamer displays high selectivity and affinity for the target cells and is greatly enriched for phenol-modified dU nucleotides (dUy, 47.5%). When the same sequences are synthesized with TTP, no binding is observed. Taken together, these findings highlight the value of using modified nucleotide triphosphates in aptamer selections and portends success in SELEX against an array of whole cells as targets.

Graphical abstract: Whole cell-SELEX of aptamers with a tyrosine-like side chain against live bacteria

Back to tab navigation

Supplementary files

Article information


Submitted
09 Nov 2016
Accepted
13 Dec 2016
First published
22 Dec 2016

Org. Biomol. Chem., 2017,15, 1980-1989
Article type
Paper

Whole cell-SELEX of aptamers with a tyrosine-like side chain against live bacteria

M. Renders, E. Miller, C. H. Lam and D. M. Perrin, Org. Biomol. Chem., 2017, 15, 1980
DOI: 10.1039/C6OB02451C

Social activity

Search articles by author

Spotlight

Advertisements