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Issue 1, 2017
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The 5-chlorouracil:7-deazaadenine base pair as an alternative to the dT:dA base pair

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Abstract

5-Chloro-2′-deoxyuridine as a possible component of a chemically modified genome has been discussed in terms of its influence on duplex stability and DNA polymerase incorporation properties. The search for its counterpart among different deoxyadenosine analogs (7-deaza-, 8-aza- and 8-aza-7-deaza-2′-deoxyadenosines) showed that the stable duplex formation as well as the synthesis of long constructs, more than 2 kb, were successful with the 5-chloro-2′-deoxyuridine and 7-deaza-2′-deoxyadenosine combination and with Taq DNA polymerase.

Graphical abstract: The 5-chlorouracil:7-deazaadenine base pair as an alternative to the dT:dA base pair

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Publication details

The article was received on 18 Oct 2016, accepted on 28 Nov 2016 and first published on 05 Dec 2016


Article type: Paper
DOI: 10.1039/C6OB02274J
Citation: Org. Biomol. Chem., 2017,15, 168-176
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    The 5-chlorouracil:7-deazaadenine base pair as an alternative to the dT:dA base pair

    E. Eremeeva, M. Abramov, P. Marlière and P. Herdewijn, Org. Biomol. Chem., 2017, 15, 168
    DOI: 10.1039/C6OB02274J

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