Jump to main content
Jump to site search

Issue 39, 2017
Previous Article Next Article

Nanoscale patterning of self-assembled monolayer (SAM)-functionalised substrates with single molecule contact printing

Author affiliations

Abstract

Defined arrangements of individual molecules are covalenty connected (“printed”) onto SAM-functionalised gold substrates with nanometer resolution. Substrates were initially pre-functionlised by coating with 3,3′-dithiodipropionic acid (DTPA) to form a self-assembled monolayer (SAM), which was characterised by atomic force microscopy (AFM), contact angle goniometry, cyclic voltammetry and surface plasmon resonance (SPR) spectroscopy. Pre-defined “ink” patterns displayed on DNA origami-based single-use carriers (“stamp”) were covalently conjugated to the SAM using 1-ethyl-3-(3-dimethylamino-propyl)carbodiimide (EDC) and N-hydroxy-succinimide (NHS). These anchor points were used to create nanometer-precise single-molecule arrays, here with complementary DNA and streptavidin. Sequential steps of the printing process were evaluated by AFM and SPR spectroscopy. It was shown that 30% of the detected arrangements closely match the expected length distribution of designed patterns, whereas another 40% exhibit error within the range of only 1 streptavidin molecule. SPR results indicate that imposing a defined separation between molecular anchor points within the pattern through this printing process enhances the efficiency for association of specific binding partners for systems with high sterical hindrance. This study expands upon earlier findings where geometrical information was conserved by the application of DNA nanostructures, by establishing a generalisable strategy which is universally applicable to nearly any type of prefunctionalised substrate such as metals, plastics, silicates, ITO or 2D materials.

Graphical abstract: Nanoscale patterning of self-assembled monolayer (SAM)-functionalised substrates with single molecule contact printing

Back to tab navigation

Supplementary files

Publication details

The article was received on 24 May 2017, accepted on 01 Sep 2017 and first published on 02 Oct 2017


Article type: Paper
DOI: 10.1039/C7NR03696E
Citation: Nanoscale, 2017,9, 15098-15106
  • Open access: Creative Commons BY license
  •   Request permissions

    Nanoscale patterning of self-assembled monolayer (SAM)-functionalised substrates with single molecule contact printing

    M. Sajfutdinow, K. Uhlig, A. Prager, C. Schneider, B. Abel and D. M. Smith, Nanoscale, 2017, 9, 15098
    DOI: 10.1039/C7NR03696E

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements