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Issue 19, 2017
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Site accessibility tailors DNA cleavage by restriction enzymes in DNA confined monolayers

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Abstract

Density-tunable nanografted monolayers (NAMs) of short oligonucleotide sequences on gold surfaces show novel properties that make them suitable for advanced biosensing applications, and in particular to study the effects of crowding and confinement on biomolecular interactions. Here, combining atomic force microscopy nanolithography, topography measurements and coarse-grained molecular dynamics simulations, we investigated restriction enzyme reaction mechanisms within confined DNA brushes highlighting the role played by the DNA sequence conformation and restriction site position along the chain, respectively, in determining the accessibility of the enzyme, and its consequent cleavage efficiency.

Graphical abstract: Site accessibility tailors DNA cleavage by restriction enzymes in DNA confined monolayers

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Supplementary files

Article information


Submitted
09 Feb 2017
Accepted
12 Apr 2017
First published
17 Apr 2017

Nanoscale, 2017,9, 6399-6405
Article type
Paper

Site accessibility tailors DNA cleavage by restriction enzymes in DNA confined monolayers

C. Rotella, G. Doni, A. Bosco, M. Castronovo, A. De Vita, L. Casalis, G. M. Pavan and P. Parisse, Nanoscale, 2017, 9, 6399
DOI: 10.1039/C7NR00966F

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