High throughput fabrication of cell spheroids by templating water-in-water Pickering emulsions†
Anupam A. K.
Das, 
a
Benjamin W.
Filby,
a
Daniel A.
Geddes,
a
Deborah
Legrande
a
and
Vesselin N.
Paunov
*a
a
Benjamin W.
Filby,
a
Daniel A.
Geddes,
a
Deborah
Legrande
a
and
Vesselin N.
Paunov
*a
Abstract
We report a simple technique for the high throughput generation of tissue spheroids based on the encapsulation of dispersed adherent cells in particle-stabilised water-in-water emulsions. We demonstrate cell encapsulation and spheroid generation using fibroblast cells which can find applications in 3D culturing, tissue engineering, inks for bioprinting and cell therapy.
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