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Issue 18, 2017
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Cell-free extract based optimization of biomolecular circuits with droplet microfluidics

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Abstract

Engineering an efficient biomolecular circuit often requires time-consuming iterations of optimization. Cell-free protein expression systems allow rapid testing of biocircuits in vitro, speeding the design–build–test cycle of synthetic biology. In this paper, we combine this with droplet microfluidics to densely scan a transcription–translation biocircuit space. Our system assays millions of parameter combinations per hour, providing a detailed map of function. The ability to comprehensively map biocircuit parameter spaces allows accurate modeling to predict circuit function and identify optimal circuits and conditions.

Graphical abstract: Cell-free extract based optimization of biomolecular circuits with droplet microfluidics

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Supplementary files

Article information


Submitted
23 May 2017
Accepted
27 Jul 2017
First published
03 Aug 2017

Lab Chip, 2017,17, 3037-3042
Article type
Communication

Cell-free extract based optimization of biomolecular circuits with droplet microfluidics

Y. Hori, C. Kantak, R. M. Murray and A. R. Abate, Lab Chip, 2017, 17, 3037
DOI: 10.1039/C7LC00552K

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