Jump to main content
Jump to site search

Issue 6, 2017
Previous Article Next Article

Paper-based microfluidic system for tear electrolyte analysis

Author affiliations

Abstract

The analysis of tear constituents at point-of-care settings has a potential for early diagnosis of ocular disorders such as dry eye disease, low-cost screening, and surveillance of at-risk subjects. However, current minimally-invasive rapid tear analysis systems for point-of-care settings have been limited to assessment of osmolarity or inflammatory markers and cannot differentiate between dry eye subclassifications. Here, we demonstrate a portable microfluidic system that allows quantitative analysis of electrolytes in the tear fluid that is suited for point-of-care settings. The microfluidic system consists of a capillary tube for sample collection, a reservoir for sample dilution, and a paper-based microfluidic device for electrolyte analysis. The sensing regions are functionalized with fluorescent crown ethers, o-acetanisidide, and seminaphtorhodafluor that are sensitive to mono- and divalent electrolytes, and their fluorescence outputs are measured with a smartphone readout device. The measured sensitivity values of Na+, K+, Ca2+ ions and pH in artificial tear fluid were matched with the known ion concentrations within the physiological range. The microfluidic system was tested with samples having different ionic concentrations, demonstrating the feasibility for the detection of early-stage dry eye, differential diagnosis of dry eye sub-types, and their severity staging.

Graphical abstract: Paper-based microfluidic system for tear electrolyte analysis

Back to tab navigation

Supplementary files

Publication details

The article was received on 25 Nov 2016, accepted on 13 Jan 2017 and first published on 16 Feb 2017


Article type: Paper
DOI: 10.1039/C6LC01450J
Citation: Lab Chip, 2017,17, 1137-1148
  • Open access: Creative Commons BY license
  •   Request permissions

    Paper-based microfluidic system for tear electrolyte analysis

    A. K. Yetisen, N. Jiang, A. Tamayol, G. U. Ruiz-Esparza, Y. S. Zhang, S. Medina-Pando, A. Gupta, J. S. Wolffsohn, H. Butt, A. Khademhosseini and S. Yun, Lab Chip, 2017, 17, 1137
    DOI: 10.1039/C6LC01450J

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements