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Issue 4, 2017
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One-step micromolding of complex 3D microchambers for single-cell analysis

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Abstract

Herein we examined the extent of replicability of the PDMS microchamber device transferred from the master mold with complex 3D structures fabricated via micro stereolithography. Due to the elastomeric properties of PDMS, the reversely tapered micromold, with the diameter ratio of ∼5 from the largest to the narrowest part, was precisely transferred without breaking. We obtained the mathematical model to estimate the stress exerted on the mold during the demolding process. Finally, we tested the applicability of this unusual microchamber for single-cell trapping and an enzyme assay.

Graphical abstract: One-step micromolding of complex 3D microchambers for single-cell analysis

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Supplementary files

Article information


Submitted
23 Oct 2016
Accepted
05 Jan 2017
First published
19 Jan 2017

Lab Chip, 2017,17, 647-652
Article type
Technical Innovation

One-step micromolding of complex 3D microchambers for single-cell analysis

H. Suzuki, K. Mitsuno, K. Shiroguchi, M. Tsugane, T. Okano, T. Dohi and T. Tsuji, Lab Chip, 2017, 17, 647
DOI: 10.1039/C6LC01313A

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