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Issue 4, 2017
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One-step micromolding of complex 3D microchambers for single-cell analysis

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Abstract

Herein we examined the extent of replicability of the PDMS microchamber device transferred from the master mold with complex 3D structures fabricated via micro stereolithography. Due to the elastomeric properties of PDMS, the reversely tapered micromold, with the diameter ratio of ∼5 from the largest to the narrowest part, was precisely transferred without breaking. We obtained the mathematical model to estimate the stress exerted on the mold during the demolding process. Finally, we tested the applicability of this unusual microchamber for single-cell trapping and an enzyme assay.

Graphical abstract: One-step micromolding of complex 3D microchambers for single-cell analysis

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Publication details

The article was received on 23 Oct 2016, accepted on 05 Jan 2017 and first published on 19 Jan 2017


Article type: Technical Innovation
DOI: 10.1039/C6LC01313A
Lab Chip, 2017,17, 647-652

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    One-step micromolding of complex 3D microchambers for single-cell analysis

    H. Suzuki, K. Mitsuno, K. Shiroguchi, M. Tsugane, T. Okano, T. Dohi and T. Tsuji, Lab Chip, 2017, 17, 647
    DOI: 10.1039/C6LC01313A

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