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Issue 2, 2017
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Centrifugal micro-channel array droplet generation for highly parallel digital PCR

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Abstract

Stable water-in-oil emulsion is essential to digital PCR and many other bioanalytical reactions that employ droplets as microreactors. We developed a novel technology to produce monodisperse emulsion droplets with high efficiency and high throughput using a bench-top centrifuge. Upon centrifugal spinning, the continuous aqueous phase is dispersed into monodisperse droplet jets in air through a micro-channel array (MiCA) and then submerged into oil as a stable emulsion. We performed dPCR reactions with a high dynamic range through the MiCA approach, and demonstrated that this cost-effective method not only eliminates the usage of complex microfluidic devices and control systems, but also greatly suppresses the loss of materials and cross-contamination. MiCA-enabled highly parallel emulsion generation combines both easiness and robustness of picoliter droplet production, and breaks the technical challenges by using conventional lab equipment and supplies.

Graphical abstract: Centrifugal micro-channel array droplet generation for highly parallel digital PCR

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Publication details

The article was received on 20 Oct 2016, accepted on 06 Dec 2016 and first published on 09 Dec 2016


Article type: Communication
DOI: 10.1039/C6LC01305H
Citation: Lab Chip, 2017,17, 235-240

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    Centrifugal micro-channel array droplet generation for highly parallel digital PCR

    Z. Chen, P. Liao, F. Zhang, M. Jiang, Y. Zhu and Y. Huang, Lab Chip, 2017, 17, 235
    DOI: 10.1039/C6LC01305H

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