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Issue 5, 2017
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Analytical figures of merit for a novel tissue imaging system

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Abstract

A novel laser-ablation sample introduction system has been developed for the Fluidigm® Helios™ mass cytometer to enable highly multiplexed imaging of biological samples. Herein, we report on the analytical figures of merit for this system using a slide, coated uniformly with a metal-doped PMMA film, as a test sample. The system is based on the use of a fifth harmonic Nd–YAG laser focused to a spot size of 1 micrometer or less. A fast wash-out ablation chamber provides resolution of individual plumes when operated at a 100 Hz or 200 Hz laser repetition frequency. The system exhibits a linear response to metal concentration in the sample over 4 orders of magnitude. The variation of signals generated for 1 micrometer diameter pixels is ≤15% RSD. Pixel-to-pixel sample contamination due to redeposition of the ablated material is approximately 0.6%. The variation of the ablated crater diameter with laser energy is also explored, demonstrating a linear behaviour over a limited range of laser energies.

Graphical abstract: Analytical figures of merit for a novel tissue imaging system

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Article information


Submitted
18 Nov 2016
Accepted
13 Mar 2017
First published
30 Mar 2017

J. Anal. At. Spectrom., 2017,32, 1044-1051
Article type
Technical Note

Analytical figures of merit for a novel tissue imaging system

R. N. Straus, A. Carew, D. Sandkuijl, T. Closson, V. I. Baranov and A. Loboda, J. Anal. At. Spectrom., 2017, 32, 1044
DOI: 10.1039/C6JA00420B

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