Issue 2, 2017

Microsphere cytometry to interrogate microenvironment-dependent cell signaling


Microenvironmental cues comprising surface-mediated and soluble factors control cellular signaling mechanisms underlying normal cellular responses that define homeostatic and diseased cell states. In order to measure cell signaling in single adherent cells, we developed a novel microsphere-based flow cytometry approach. Single normal or neoplastic cells were adhered to uniform microspheres that display mimetic-microenvironments comprising surface combinations of extracellular matrix (ECM) in the presence of soluble agonists/antagonists. Temporal signaling responses were measured with fluorophore-conjugated antibodies that recognize response-dependent epitopes by multiparametric flow cytometry. Using this approach we demonstrate that microenvironment-mimetic combinations of growth factors and extracellular matrix proteins generate distinct cellular signal networks that reveal unique cell signatures in normal and patient biopsy-derived neoplastic cells.

Graphical abstract: Microsphere cytometry to interrogate microenvironment-dependent cell signaling

Supplementary files

Article information

Article type
04 Oct 2016
04 Jan 2017
First published
09 Jan 2017

Integr. Biol., 2017,9, 123-134