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Volume 198, 2017
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13C-Labeling the carbon-fixation pathway of a highly efficient artificial photosynthetic system

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Abstract

Interfacing the CO2-fixing microorganism, Ralstonia eutropha, to the energy derived from hydrogen produced by water splitting is a viable approach to achieving renewable CO2 reduction at high efficiencies. We employ 13C-labeling to report on the nature of CO2 reduction in the inorganic water splitting|R. eutropha hybrid system. Accumulated biomass in a reactor under a 13C-enriched CO2 atmosphere may be sampled at different time points during CO2 reduction. Converting the sampled biomass into gaseous CO2 allows the 13C/12C ratio to be determined by gas chromatography-mass spectrometry. After 2 hours of inoculation and the initiation of water splitting, the microbes adapted and began to convert CO2 into biomass. The observed time evolution of the 13C/12C ratio in accumulated biomass is consistent with a Monod model for carbon fixation. Carbon dioxide produced by catabolism was found to be minimal. This rapid response of the bacteria to a hydrogen input and to subsequent CO2 reduction at high efficiency are beneficial to achieving artificial photosynthesis for the storage of renewable energy.

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Article information


Submitted
17 Nov 2016
Accepted
22 Nov 2016
First published
22 Nov 2016

Faraday Discuss., 2017,198, 529-537
Article type
Paper

13C-Labeling the carbon-fixation pathway of a highly efficient artificial photosynthetic system

C. Liu, S. N. Nangle, B. C. Colón, P. A. Silver and D. G. Nocera, Faraday Discuss., 2017, 198, 529
DOI: 10.1039/C6FD00231E

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