Issue 36, 2017

Photoinduced dimerization of a photosensory DNA-binding protein EL222 and its LOV domain

Abstract

EL222 is a blue light sensor protein consisting of a light-oxygen-voltage (LOV) domain (EL-LOV domain) at the N-terminus and a helix-turn-helix DNA-binding domain at the C-terminus. EL222 acts as a light dependent transcriptional factor. The photochemical reactions of EL222 and the light sensing properties of the LOV domain were investigated. Concentration dependent experiments revealed that the EL-LOV domain is in equilibrium between the dimer and the monomer in the dark state, and the main photoreaction is the dimerization reaction between a monomer in the ground state and that in the excited state. The equilibrium constant and the intrinsic rate constants of dimerization were determined. EL222 was found to also exhibit photoinduced dimerization even in the absence of target DNA, although the yield of the reaction was low (∼0.08 compared with that of the EL-LOV domain). This observation suggests that there are inhomogeneous conformations, open and closed types, of EL222 in solution.

Graphical abstract: Photoinduced dimerization of a photosensory DNA-binding protein EL222 and its LOV domain

Supplementary files

Article information

Article type
Paper
Submitted
01 Jun 2017
Accepted
18 Aug 2017
First published
18 Aug 2017

Phys. Chem. Chem. Phys., 2017,19, 24855-24865

Photoinduced dimerization of a photosensory DNA-binding protein EL222 and its LOV domain

A. Takakado, Y. Nakasone and M. Terazima, Phys. Chem. Chem. Phys., 2017, 19, 24855 DOI: 10.1039/C7CP03686H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements