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Issue 20, 2017
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Formation of modulated phases and domain rigidification in fatty acid-containing lipid membranes

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Abstract

We investigated the phase behavior of lipid membranes containing fatty acids (FAs) by microscopy and differential scanning calorimetry. We used palmitic acid (saturated FA), oleic acid (cis-isomer of unsaturated FA), elaidic acid (trans-isomer of unsaturated FA), and phytanic acid (branched FA) and examined the effects of FAs on phase-separated structures in lipid bilayer membranes consisting of dioleolylphosphocholine (DOPC)/dipalmitoylphosphocholine (DPPC)/cholesterol (Chol). Palmitic acid and elaidic acid exclude Chol from the DPPC-rich phase. As a result, the liquid-ordered phase formed by DPPC and Chol transforms into a solid-ordered phase. Oleic acid and phytanic acid significantly reduce the line tension at the liquid domain boundary. This decrease in line tension leads to the formation of modulated phases, such as striped, hexagonal, and polygonal domains. We measured the line tension and the interdomain interaction in these specific domains by an image analysis. The result showed that oleic acid and phytanic acid-containing vesicles as well as palmitic acid-containing vesicles are not spherical, and this domain-induced deformation is explained theoretically.

Graphical abstract: Formation of modulated phases and domain rigidification in fatty acid-containing lipid membranes

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Article information


Submitted
23 Feb 2017
Accepted
20 Apr 2017
First published
24 Apr 2017

Phys. Chem. Chem. Phys., 2017,19, 13252-13263
Article type
Paper

Formation of modulated phases and domain rigidification in fatty acid-containing lipid membranes

N. Shimokawa, R. Mukai, M. Nagata and M. Takagi, Phys. Chem. Chem. Phys., 2017, 19, 13252
DOI: 10.1039/C7CP01201B

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