Issue 96, 2017

Proton-detected solid-state NMR detects the inter-nucleotide correlations and architecture of dimeric RNA in microcrystals

Abstract

We report a novel proton-detected MAS solid-state NMR strategy based on 15N–15N proton assisted recoupling to detect the inter-nucleotide NH⋯N hydrogen bonds within the Watson–Crick base pairs of micro-crystallized dimeric RNA and to confirm the kissing-loop structure. This would contribute to advances in the structural determination of RNA using solid-state NMR.

Graphical abstract: Proton-detected solid-state NMR detects the inter-nucleotide correlations and architecture of dimeric RNA in microcrystals

Supplementary files

Article information

Article type
Communication
Submitted
25 Sep 2017
Accepted
26 Oct 2017
First published
26 Oct 2017

Chem. Commun., 2017,53, 12886-12889

Proton-detected solid-state NMR detects the inter-nucleotide correlations and architecture of dimeric RNA in microcrystals

Y. Yang, S. Xiang, X. Liu, X. Pei, P. Wu, Q. Gong, N. Li, M. Baldus and S. Wang, Chem. Commun., 2017, 53, 12886 DOI: 10.1039/C7CC07483B

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