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Issue 97, 2017
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Facile immobilization of DNA using an enzymatic his-tag mimic

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Abstract

Methods for immobilization of DNA on solid supports are in high demand. Herein, we present a generally applicable enzymatic method for the immobilization of DNA without any prior chemical derivatization. This strategy relies on the homopolymerization of the modified triphosphate dImTP by the TdT. The resulting enzymatic his-tag mimic ensures binding of DNA on Ni-NTA agarose. The usefulness of this method is highlighted by the immobilization of functional nucleic acids without impairing their specific activities.

Graphical abstract: Facile immobilization of DNA using an enzymatic his-tag mimic

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Publication details

The article was received on 14 Sep 2017, accepted on 14 Nov 2017 and first published on 22 Nov 2017


Article type: Communication
DOI: 10.1039/C7CC07207D
Chem. Commun., 2017,53, 13031-13034

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    Facile immobilization of DNA using an enzymatic his-tag mimic

    P. Röthlisberger, F. Levi-Acobas, I. Sarac, B. Baron, P. England, P. Marlière, P. Herdewijn and M. Hollenstein, Chem. Commun., 2017, 53, 13031
    DOI: 10.1039/C7CC07207D

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