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Issue 87, 2017
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Target and identify: triazene linker helps identify azidation sites of labelled proteins via click and cleave strategy

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Abstract

A method for identifying probe modification of proteins via tandem mass spectrometry was developed. Azide bearing molecules are immobilized on functionalised sepharose beads via copper catalysed Huisgen-type click chemistry and selectively released under acidic conditions by chemical cleavage of the triazene linkage. We applied this method to identify the modification site of targeted-diazotransfer on BirA.

Graphical abstract: Target and identify: triazene linker helps identify azidation sites of labelled proteins via click and cleave strategy

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Publication details

The article was received on 06 Sep 2017, accepted on 11 Oct 2017 and first published on 16 Oct 2017


Article type: Communication
DOI: 10.1039/C7CC07001B
Citation: Chem. Commun., 2017,53, 11929-11932

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    Target and identify: triazene linker helps identify azidation sites of labelled proteins via click and cleave strategy

    J. Lohse, A. Schindl, N. Danda, C. P. Williams, K. Kramer, B. Kuster, M. D. Witte and G. Médard, Chem. Commun., 2017, 53, 11929
    DOI: 10.1039/C7CC07001B

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