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Issue 96, 2017
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Chemical synthesis and NMR spectroscopy of long stable isotope labelled RNA

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Abstract

We showcase the high potential of the 2′-cyanoethoxymethyl (CEM) methodology to synthesize RNAs with naturally occurring modified residues carrying stable isotope (SI) labels for NMR spectroscopic applications. The method was applied to synthesize RNAs with sizes ranging between 60 to 80 nucleotides. The presented approach gives the possibility to selectively modify larger RNAs (>60 nucleotides) with atom-specifically 13C/15N-labelled building blocks. The method harbors the unique potential to address structural as well as dynamic features of these RNAs with NMR spectroscopy but also using other biophysical methods, such as mass spectrometry (MS), or small angle neutron/X-ray scattering (SANS, SAXS).

Graphical abstract: Chemical synthesis and NMR spectroscopy of long stable isotope labelled RNA

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Supplementary files

Article information


Submitted
29 Aug 2017
Accepted
28 Sep 2017
First published
20 Nov 2017

This article is Open Access

Chem. Commun., 2017,53, 12938-12941
Article type
Communication

Chemical synthesis and NMR spectroscopy of long stable isotope labelled RNA

J. Kremser, E. Strebitzer, R. Plangger, M. A. Juen, F. Nußbaumer, H. Glasner, K. Breuker and C. Kreutz, Chem. Commun., 2017, 53, 12938
DOI: 10.1039/C7CC06747J

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