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Issue 88, 2017
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Debranchase-resistant labeling of RNA using the 10DM24 deoxyribozyme and fluorescent modified nucleotides

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Abstract

The 10DM24 deoxyribozyme can site-specifically label RNAs with fluorophore-GTP conjugates; however, the 2′,5′-branched RNA linkage is readily cleaved by debranchase. To prevent loss of labels upon cleavage, we synthesized phosphorothioate-modified, fluorescent GTP derivatives and elaborated conditions for their incorporation by 10DM24. RNAs labeled with fluorescent derivatives of Sp-GTPS were found to be resistant to debranchase.

Graphical abstract: Debranchase-resistant labeling of RNA using the 10DM24 deoxyribozyme and fluorescent modified nucleotides

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Supplementary files

Article information


Submitted
26 Aug 2017
Accepted
29 Sep 2017
First published
29 Sep 2017

Chem. Commun., 2017,53, 11992-11995
Article type
Communication

Debranchase-resistant labeling of RNA using the 10DM24 deoxyribozyme and fluorescent modified nucleotides

T. J. Carrocci, L. Lohe, M. J. Ashton, C. Höbartner and A. A. Hoskins, Chem. Commun., 2017, 53, 11992
DOI: 10.1039/C7CC06703H

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