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Issue 91, 2017
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Genetic alphabet expansion transcription generating functional RNA molecules containing a five-letter alphabet including modified unnatural and natural base nucleotides by thermostable T7 RNA polymerase variants

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Abstract

Thermostable T7 RNA polymerase variants were explored for genetic alphabet expansion transcription involving the unnatural Ds–Pa pair. One variant exhibited high incorporation efficiencies of functionally modified Pa substrates and enabled the simultaneous incorporation of 2′-fluoro-nucleoside triphosphates of pyrimidines into transcripts, allowing the generation of novel, highly functional RNA molecules.

Graphical abstract: Genetic alphabet expansion transcription generating functional RNA molecules containing a five-letter alphabet including modified unnatural and natural base nucleotides by thermostable T7 RNA polymerase variants

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Publication details

The article was received on 25 Aug 2017, accepted on 25 Oct 2017 and first published on 25 Oct 2017


Article type: Communication
DOI: 10.1039/C7CC06661A
Citation: Chem. Commun., 2017,53, 12309-12312

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    Genetic alphabet expansion transcription generating functional RNA molecules containing a five-letter alphabet including modified unnatural and natural base nucleotides by thermostable T7 RNA polymerase variants

    M. Kimoto, A. J. Meyer, I. Hirao and A. D. Ellington, Chem. Commun., 2017, 53, 12309
    DOI: 10.1039/C7CC06661A

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