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Issue 80, 2017
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One-step detection of microRNA with high sensitivity and specificity via target-triggered loop-mediated isothermal amplification (TT-LAMP)

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Abstract

A novel one-step microRNA assay is developed based on a target-triggered loop-mediated isothermal amplification (TT-LAMP) mechanism, which enables the accurate detection of as low as 100 aM (1 zmol) microRNA with simple one-step operation by using only one-type of DNA polymerase.

Graphical abstract: One-step detection of microRNA with high sensitivity and specificity via target-triggered loop-mediated isothermal amplification (TT-LAMP)

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Article information


Submitted
06 Aug 2017
Accepted
18 Sep 2017
First published
18 Sep 2017

Chem. Commun., 2017,53, 11040-11043
Article type
Communication

One-step detection of microRNA with high sensitivity and specificity via target-triggered loop-mediated isothermal amplification (TT-LAMP)

Y. Sun, H. Tian, C. Liu, Y. Sun and Z. Li, Chem. Commun., 2017, 53, 11040
DOI: 10.1039/C7CC06140D

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