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Abstract | Cited by

We successfully converted an antibody single-chain variable fragment and a full-sized antibody to Quenchbodies, which are a type of powerful fluorescent immunosensor, through ultraviolet-based photochemical crosslinking of an indole-3-butyric acid-conjugated fluorescent dye to the nucleotide-binding sites near the antigen-binding sites.

Graphical abstract: Construction of dye-stapled Quenchbodies by photochemical crosslinking to antibody nucleotide-binding sites

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