Issue 57, 2017

Real-time activity monitoring of New Delhi metallo-β-lactamase-1 in living bacterial cells by UV-Vis spectroscopy

Abstract

We report an UV-Vis method for monitoring the hydrolysis of the β-lactam antibiotics inside living bacterial cells. Cell-based studies demonstrated that the hydrolysis of cefazolin was inhibited by three known NDM-1 inhibitors. This approach can be applied to the monitoring of reactions in a complex biological system, for instance in medical testing.

Graphical abstract: Real-time activity monitoring of New Delhi metallo-β-lactamase-1 in living bacterial cells by UV-Vis spectroscopy

Supplementary files

Article information

Article type
Communication
Submitted
13 Apr 2017
Accepted
22 Jun 2017
First published
22 Jun 2017

Chem. Commun., 2017,53, 8014-8017

Real-time activity monitoring of New Delhi metallo-β-lactamase-1 in living bacterial cells by UV-Vis spectroscopy

K. Yang, Y. Zhou, Y. Ge and Y. Zhang, Chem. Commun., 2017, 53, 8014 DOI: 10.1039/C7CC02774E

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