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Issue 48, 2017
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A wash-free SNAP-tag fluorogenic probe based on the additive effects of quencher release and environmental sensitivity

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Abstract

A 1,8-naphthalimide-derived fluorogenic probe was reported to label SNAP-tag fusion proteins in living cells. The probe can rapidly label a SNAP-tag and exhibit a fluorescence increase of 36-fold due to the additive effects of environment sensitivity of fluorophores and inhibition of photo-induced electron transfer from O6-benzylguanine to the fluorophore. The labeling of intracellular proteins has been successfully achieved without a wash-out procedure.

Graphical abstract: A wash-free SNAP-tag fluorogenic probe based on the additive effects of quencher release and environmental sensitivity

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Supplementary files

Article information


Submitted
02 Mar 2017
Accepted
23 May 2017
First published
23 May 2017

Chem. Commun., 2017,53, 6448-6451
Article type
Communication

A wash-free SNAP-tag fluorogenic probe based on the additive effects of quencher release and environmental sensitivity

S. Leng, Q. Qiao, L. Miao, W. Deng, J. Cui and Z. Xu, Chem. Commun., 2017, 53, 6448
DOI: 10.1039/C7CC01483J

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