Reversible DNA micro-patterning using the fluorous effect

Gabriella E. Flynn; Jamie M. Withers; Gerard Macias; Justin R. Sperling; Sarah L. Henry; Jonathan M. Cooper; Glenn A. Burley; Alasdair W. Clark

doi:10.1039/C7CC00288B

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Reversible DNA micro-patterning using the fluorous effect†

Gabriella E. Flynn,

^a Jamie M. Withers,^b Gerard Macias,^a Justin R. Sperling,^a Sarah L. Henry,^a Jonathan M. Cooper,^a Glenn A. Burley

*^b and Alasdair W. Clark*^a

Author affiliations

* Corresponding authors

^a Biomedical Engineering Research Division, School of Engineering, University of Glasgow, Rankine Building, Oakfield Avenue, Glasgow, UK
E-mail: Alasdair.clark@glasgow.ac.uk

^b WestCHEM & Department of Pure & Applied Chemistry, University of Strathclyde, 295 Cathedral Street, Glasgow, UK
E-mail: glenn.burley@strath.ac.uk

Abstract

We describe a new method for the immobilisation of DNA into defined patterns with sub-micron resolution, using the fluorous effect. The method is fully reversible via a simple solvent wash, allowing the patterning, regeneration and re-patterning of surfaces with no degradation in binding efficiency following multiple removal/attachment cycles of different DNA sequences.

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Article information

DOI: https://doi.org/10.1039/C7CC00288B
Article type: Communication
Submitted: 12 Jan 2017
Accepted: 15 Feb 2017
First published: 23 Feb 2017
This article is Open Access

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Chem. Commun., 2017,53, 3094-3097

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Reversible DNA micro-patterning using the fluorous effect

G. E. Flynn, J. M. Withers, G. Macias, J. R. Sperling, S. L. Henry, J. M. Cooper, G. A. Burley and A. W. Clark, Chem. Commun., 2017, 53, 3094 DOI: 10.1039/C7CC00288B

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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Chemical Communications

Reversible DNA micro-patterning using the fluorous effect†

Abstract

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Reversible DNA micro-patterning using the fluorous effect

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