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Issue 5, 2017
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Intrinsic blinking of red fluorescent proteins for super-resolution microscopy

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Abstract

Single-molecule localization microscopy relies on either controllable photoswitching of fluorescent probes or their robust blinking. We have found that blinking of monomeric red fluorescent proteins TagRFP, TagRFP-T, and FusionRed occurs at moderate illumination power and matches well with camera acquisition speed. It allows for super-resolution image reconstruction of densely labelled structures in live cells using various algorithms.

Graphical abstract: Intrinsic blinking of red fluorescent proteins for super-resolution microscopy

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Publication details

The article was received on 17 Nov 2016, accepted on 19 Dec 2016 and first published on 19 Dec 2016


Article type: Communication
DOI: 10.1039/C6CC09200D
Chem. Commun., 2017,53, 949-951

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    Intrinsic blinking of red fluorescent proteins for super-resolution microscopy

    N. V. Klementieva, A. I. Pavlikov, A. A. Moiseev, N. G. Bozhanova, N. M. Mishina, S. A. Lukyanov, E. V. Zagaynova, K. A. Lukyanov and A. S. Mishin, Chem. Commun., 2017, 53, 949
    DOI: 10.1039/C6CC09200D

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