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Abstract | Cited by

Here we present mGarnet2, a monomeric, far-red fluorescent marker protein derived from mRuby, with absorption and emission bands peaking at 598 and 671 nm, respectively. The protein shows excellent performance as a live-cell fusion marker for STED nanoscopy with 640 nm excitation and 780 nm depletion wavelengths.

Graphical abstract: A far-red emitting fluorescent marker protein, mGarnet2, for microscopy and STED nanoscopy

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