Issue 9, 2017

Synchronization of excitable cardiac cultures of different origin


In the present work, we investigated the synchronization of electrical activity in cultured cardiac cells of different origin put in direct contact. In the first set of experiments synchronization was studied in the primary culture cells of neonatal rats taken at different developmental ages, and in the second – in the neonatal rat cardiomyocytes and HL-1 cells. The electrical excitation of cells was recorded using the calcium transient marker Fluor-4. In the confluent cell layers created with the aid of a specially devised mask, the excitation waves and their propagation between areas occupied by cells of different origin were observed. On the level of individual cells, their contact and synchronization was monitored with the aid of scanning fluorescence microscopy. It was found that populations of cultured cells of different origin are able to synchronize, suggesting the formation of electrical coupling between them. The results obtained may be considered as a proof of concept that implanted alien grafted cells are able to create electrical coupling with the host cardiac tissue.

Graphical abstract: Synchronization of excitable cardiac cultures of different origin

Article information

Article type
02 Mar 2017
06 Jun 2017
First published
07 Jun 2017
This article is Open Access
Creative Commons BY license

Biomater. Sci., 2017,5, 1777-1785

Synchronization of excitable cardiac cultures of different origin

N. N. Agladze, O. V. Halaidych, V. A. Tsvelaya, T. Bruegmann, C. Kilgus, P. Sasse and K. I. Agladze, Biomater. Sci., 2017, 5, 1777 DOI: 10.1039/C7BM00171A

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