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Issue 40, 2017
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Ligand identification of the adenosine A2A receptor in self-assembled nanodiscs by affinity mass spectrometry

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Abstract

A nanodisc is designed as a vehicle to contain GPCRs in a native-like phospholipid environment. It maintains GPCRs in a better thermostability and homogeneity state in a detergent-free buffer compared to a detergent micelle environment. Here, we established an affinity mass spectrometry-based approach to assay the interaction between the adenosine A2A receptor in the nanodisc and eight known ligands. The affinity mass spectrometry results are consistent with the previously published data for binding affinities. The combination of nanodisc and affinity MS techniques allows the identification of the GPCR ligands in a relatively stable, unliganded/apo and native state.

Graphical abstract: Ligand identification of the adenosine A2A receptor in self-assembled nanodiscs by affinity mass spectrometry

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Supplementary files

Article information


Submitted
03 Aug 2017
Accepted
18 Sep 2017
First published
12 Oct 2017

Anal. Methods, 2017,9, 5851-5858
Article type
Paper

Ligand identification of the adenosine A2A receptor in self-assembled nanodiscs by affinity mass spectrometry

J. Ma, Y. Lu, D. Wu, Y. Peng, W. Loa-Kum-Cheung, C. Peng, R. J. Quinn, W. Shui and Z. Liu, Anal. Methods, 2017, 9, 5851
DOI: 10.1039/C7AY01891F

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