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Issue 31, 2017

An electrochemical aptasensor for detection of bovine interferon gamma

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Abstract

Bovine tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (MTB) in which it is hard to identify the pathological symptoms. Release of bovine interferon gamma (BoIFN-γ) by T-cells provides an important diagnostic marker of MTB infections. In this work, we developed for the first time an electrochemical aptasensor for sensitive and specific determination of BoIFN-γ. A thiolated IFN-γ-binding aptamer was conjugated with methylene blue (MB) and immobilized on a gold electrode by self-assembly. Binding of IFN-γ to the electrode surface caused a conformational change in the aptamer, decreasing electron transfer efficiency. The redox current was quantified using square wave voltammetry (SWV) and was found to be specific for bovine IFN-γ with detection limit of 0.1 nM in pristine buffer and 0.9 nM in blood. The biosensor described here may, in the future, be used for on-site testing of bovine blood to help better identify and contain outbreaks of bovine TB.

Graphical abstract: An electrochemical aptasensor for detection of bovine interferon gamma

Article information


Submitted
26 May 2017
Accepted
10 Jul 2017
First published
17 Jul 2017

Anal. Methods, 2017,9, 4527-4532
Article type
Paper

An electrochemical aptasensor for detection of bovine interferon gamma

B. P. Crulhas, D. Hadley, Y. Liu, D. Shin, G. Stybayeva, M. Imanbekova, A. E. Hill, V. Pedrosa and A. Revzin, Anal. Methods, 2017, 9, 4527 DOI: 10.1039/C7AY01313B

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