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Issue 23, 2017
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Imaging acetaldehyde formation during ethanol metabolism in living cells using a hydrazinyl naphthalimide fluorescent probe

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Abstract

The metabolism of ethanol to acetaldehyde has been visualized in living lung epithelial cells using a hydrazinyl naphthalimide fluorescent probe. Utilizing a condensation reaction between carbonyls and a hydrazine moiety, we demonstrate that the fluorescent probe (aldehydefluor-1) AF1 reacts with a range of reactive carbonyl species including formaldehyde, acetaldehyde, glyoxylic acid, and methyl glyoxal. With AF1, it is possible to directly visualize endogenous carbonyl metabolites. Here, we have applied it towards the visualization of acetaldehyde generated from alcohol dehydrogenase mediated ethanol metabolism, validating it as a useful tool to study the roles of alcohol in respiratory disease and other pathological mechanisms.

Graphical abstract: Imaging acetaldehyde formation during ethanol metabolism in living cells using a hydrazinyl naphthalimide fluorescent probe

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Supplementary files

Article information


Submitted
15 May 2017
Accepted
27 May 2017
First published
29 May 2017

Anal. Methods, 2017,9, 3418-3421
Article type
Communication

Imaging acetaldehyde formation during ethanol metabolism in living cells using a hydrazinyl naphthalimide fluorescent probe

A. G. Reeves, M. Subbarao and A. R. Lippert, Anal. Methods, 2017, 9, 3418
DOI: 10.1039/C7AY01238A

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